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KMID : 1034820070030040267
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Molecular & Cellular Toxicology
2007 Volume.3 No. 4 p.267 ~ p.272
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Molecular Parameters for Assessing Marine Biotoxicity: Gene Expressions of Rockfish (Sebastes schlegeli) Exposed to Polycyclic Aromatic Hydrocarbons
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Woo Seon-Ock
Yum Seung-Shic
Park Hong-Seog
Jung Jee-Hyung
Lee Suk-Chan
Kim So-Jung
Lee Taek-Kyun
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Abstract
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Environmental and anthropogenic changes affect the health and stability of marine ecosystem. In this study we aimed to identify molecular biomarkers for ecotoxicological pollutants risk assessment in the rockfish (Sebastes schlegeli). We designed primers based on conserved sequences by multiple alignments of target genes from related species, and cloned the partial cDNAs of cytochrome P450 (CYP1A1), glutathione S-transferase (GST), metallothionein (MT), superoxide dismutase (SOD), ubiquitin (UB), vitellogenin (VTG) and -actin by reverse transcription polymerase chain reaction (RT-PCR) from S. schlegeli. Northern blot results indicated that these six genes expressions were significantly induced by benzo[a]pyrene (BaP, 1 ) and that the level of each of their transcripts increased in BaP-exposed rockfish in a time-dependent manner. This study suggests that transcriptional changes in these six genes may be used for monitoring environmental exposure to polycyclic aromatic hydrocarbons (PAHs).
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KEYWORD
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Sebastes schlegeli, Environmental stress, Benzo[a]pyrene, Biomarkers, Gene expression
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