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KMID : 1034820080040010072
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Molecular & Cellular Toxicology
2008 Volume.4 No. 1 p.72 ~ p.77
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The Inhibitory Effect of Pioglitazone on Agonist-dependent Vascular Contractility
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Je Hyun-Dong
Cha Sung-Jae
Jeong Ji-Hoon
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Abstract
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The present study was undertaken to determine whether pioglitazone treatment influences on the agonist-induced vascular smooth muscle contraction and, if so, to investigate the related mechanism. The measurement of isometric contractions using a computerized data acquisition system was combined with molecular experiments. Pioglitazone decreased Rho-kinase activating agonist-induced contraction but not phorbol ester-induced contraction suggesting the least involvement of -independent thin filament regulation of contractility. Furthermore, pioglitazone decreased thromboxane mimeticinduced phosphorylation of MYPT1 at Thr855, the newly-highlighted site, instead of Thr696. In conclusion, this study provides the evidence and possible related mechanism concerning the vasorelaxing effect of pioglitazone as an antihypertensive on the agonist-induced contraction in rat aortic rings regardless of endothelial function.
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KEYWORD
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MYPT1, Phorbol ester, Pioglitazone, Rhokinase, Thromboxane mimetic
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