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KMID : 1034820080040020132
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Molecular & Cellular Toxicology
2008 Volume.4 No. 2 p.132 ~ p.137
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Gene Expression Profile in Iprobenfos Exposed Medaka Fish by Microarray Analysis
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Woo Seon-Ock
Son Sung-Hee
Ryu Jae-Chun
Yum Seung-Shic
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Abstract
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Differential gene expression profiling was carried out in the hepatic tissue of medaka fish, Oryzias latipes, after exposure to an organophosphorus pesticide (OPP), Iprobenfos (IBP), a widely used pesticide in agri- and fish-culture, using a medaka cDNA micro array. Twenty six kinds of differentially expressed candidate genes, with 15 and 11 induced and repressed in their gene expressions, respectively, were associated with cytoskeleton (3.8%), development (7.7%), immune (7.7%), metabolism (30.8%), nucleic acid/protein binding (42.3%) and reproduction (7.7%). Of these genes, changes at the transcription level of five were re-evaluated by real-time quantitative PCR (qRT-PCR). Considering the known function of authentic genes, the effects of IBP on the biological activity and pathological aspects in medaka fish were discussed. The identified genes could be used as molecular biomarkers for biological responses to OPPs contamination in an aquatic environment.
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KEYWORD
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Oryzias latipes, Organophosphoruos pesticide (OPP), Iprobenfos (IBP), Differential gene expression profile, Real-time quantitative PCR, Microarray
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