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KMID : 1034820080040030211
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Molecular & Cellular Toxicology
2008 Volume.4 No. 3 p.211 ~ p.217
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Measurement of DNA Damage with Fpg/Endo III FLARE Assay and Real Time RT-PCR in SD Rats Exposed to Cumene
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Kim Soo-Jin
Rim Kyung-Taek
Kim Hyeon-Young
Lee Seong-Bae
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Abstract
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To clarify the DNA damage from reactive oxygen species, we measured the DNA damage through Fpg/Endo III FLARE (Fragment Length Analysis with Repair Enzyme) assay and real time RT-PCR. The 80 SD rats assigned to 4 dose groups exposed to cumene vapor for 90 days. With Fpg/Endo III FLARE assay in hepatocytes, we found the OTM (Olive Tail Moment) and TL (Tail Length) significantly increased in no-enzyme treated and Fpg-treated control and 8 ppm groups with 28 days exposure. In Endo III-treated 8 ppm group, significantly increased the values with 90 days exposure. With lymphocytes, it was founded the values significantly increased in no-enzyme treated 800 ppm group in 28 and 90 days. It was significantly increased in Endo III-treated 80 ppm for 28 days and 800 ppm for 90 days. From the above findings, FLARE assay was suggested as being available as a biological marker for DNA damage induced by cumene exposure in SD rats. And we used real time RT-PCR for the OGG1 mRNA expression, it had dose-dependent biologic effects in 1 day exposure, but decrease the levels of rOGG1 mRNA. Our findings provide evidence that cumene exposure may cause suppression of rOGG1 in the rat hepatocytes or lymphocytes.
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KEYWORD
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Cumene, Fpg/Endo III FLARE assay, Real time RT-PCR, OGG1
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