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KMID : 1034820080040030224
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Molecular & Cellular Toxicology
2008 Volume.4 No. 3 p.224 ~ p.229
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Eucommia ulmoides Extract Stimulates Glucose Uptake through PI 3-kinase Mediated Pathway in L6 Rat Skeletal Muscle Cells
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Hong Eui-Jae
Hong Seung-Jae
Jung Kyung-Hee
Ban Ju-Yeon
Baek Yong-Hyeon
Woo Hyun-Su
Park Dong-Suk
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Abstract
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Eucommia ulmoides (Duchung) is commonly used for treatment of diabetes in Korean traditional medicine. However, the exact mechanism of its anti-diabetic effect has not yet been fully elucidated. In this study, the effect of E. ulmoides extract on glucose uptake was investigated in L6 rat skeletal muscle cells. E. ulmoides extract stimulated the activity of phosphatidylinositol (PI) 3-kinase that is a major regulatory molecule in glucose uptake pathway. Protein kinase B (PKB) and protein kinase C- (PKC-), downstream mediators of PI 3-kinase, were also activated by E. ulmoides extract. We assessed the activity of AMP-activated protein kinase (AMPK), another regulatory molecule in glucose uptake pathway. Phosphorylation level of AMPK did not change with treatment of E. ulmoides extract. Phosphorylations of p38 mitogen activated protein kinase (p38 MAPK) and acetyl-CoA carboxylase (ACC), downstream mediators of AMPK, were not significantly different. Taken together, our results suggest that E. ulmoides may stimulate glucose uptake through PI 3-kinase but not AMPK in L6 skeletal muscle cells.
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KEYWORD
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Eucommia ulmoides, Glucose uptake, Phosphatidylinositol (PI) 3-kinase, Protein kinase B, Protein kinase C-
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