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KMID : 1034820080040040293
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Molecular & Cellular Toxicology
2008 Volume.4 No. 4 p.293 ~ p.299
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Differentially Expressed Genes in Marine Medaka Fish (Oryzias javanicus) Exposed to Cadmium
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Woo Seon-Ock
Son Sung-Hee
Park Hong-Seog
Vulpe Chris D.
Ryu Jae-Chun
Yum Seung-Shic
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Abstract
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To screen the differentially expressed genes in cadmuim-exposed marine medaka fish (Oryzias javanicus), a candidate marine test fish for ecological toxicity, the differential display polymerase chain reaction (DD-PCR) was carried out, since the genome-wide gene expression data are not available in this fish species yet. A total of 35 clones were isolated from cadmium-exposed fish and their nucleotide sequences were analyzed. The differentially expressed gene candidates were categorized to response to stimulus (3); ion binding (3); DNA binding (1); protein binding (6); carbohydrate binding (1); metabolic process (4); biological regulation (3); cellular process (2); protein synthesis (2); catalytic activity (2); sense of sight (1); immune (1); neurohormone (1); signaling activity (1); electron carrier activity (1) and others (3). For real-time quantitative RT-PCR, we selected catalase, glucose-6-phosphate dehydrogenase, heat shock protein 70, and metallothionein and confirmed that cadmium exposure enhanced induction of these four genes.
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KEYWORD
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Oryzias javanicus, Cadmium, Differential gene expression, Biomarker, Environmental stress
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