|
KMID : 1034820090050010001
|
|
Molecular & Cellular Toxicology
2009 Volume.5 No. 1 p.1 ~ p.6
|
|
|
Genome Wide Expression Profile of Agrimonia pilosa in LPS-stimulated BV-2 Microglial Cells
|
|
Sohn Sung-Hwa
Ko Eun-Jung
Kim Sung-Hoon
Kim Yang-Seok
Shin Min-Kyu
Hong Moo-Chang
Bae Hyun-Su
|
|
|
Abstract
|
|
|
|
Microglial cells constitute the first line of defense against infection and injury in the brain. This study was conducted to evaluate the protective mechanisms of Agrimonia pilosa (AP) on LPS-induced activation of BV-2 microglial cells. The effects of AP on gene expression profiles in activated BV-2 microglial cells were evaluated using microarray analysis. BV-2 microglial cells were cultured in a 100 mm dish () for 24 hr and then pretreated with 1 g/mL AP or left untreated for 30 min. Next, 1 g/mL LPS was added to the samples and the cells were reincubated at for 30 min, 3 hr and 6 hr. The gene expression profiles of the BV-2 microglial cells varied depending on the AP. The microarray analysis revealed that MAPK signaling pathway-related genes were down-regulated and IL10 gene was up-regulated in AP-treated BV-2 microglial cells. AP can affect the inflammatory response and MAPK pathway in BV-2 microglial cells.
|
|
|
KEYWORD
|
|
|
LPS, Agrimonia pilosa, Gene expression profile, Microarray
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|