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KMID : 1034820090050010044
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Molecular & Cellular Toxicology
2009 Volume.5 No. 1 p.44 ~ p.50
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Expression Profile of Inflammatory Genes in Human Airway Epithelial A549 Cells
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Sohn Sung-Hwa
Ko Eun-Jung
Kim Sung-Hoon
Kim Yang-Seok
Shin Min-Kyu
Hong Moo-Chang
Bae Hyun-Su
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Abstract
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This study was conducted to evaluate the inflammation mechanisms of tumor necrosis factor- (TNF-), interleukin-4 (IL-4), and IL--induced stimulation of A549 human epithelial cells. In the present study, A549 cells were stimulated with TNF-, IL-4 and IL- to induce expression of chemokines and adhesion molecules involved in eosinophil chemotaxis. The effects of TNF-, IL-4 and IL- on gene expression profiles in A549 cells were evaluated by oligonucleotide microarray and Real time RT-PCR. The gene expression profiles for the A549 cells varied depending on the cytokines. Also, the results of the microarray and Real time RT-PCR revealed that inflammatory-related genes were up-regulated in cytokine stimulated A549 cells. Cytokines can affect inflammation in A549 cells. A microarray-based genomic survey is a high-throughput approach that enables evaluation of gene expression in cytokine stimulated cell lines.
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KEYWORD
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Asthma, Gene expression profile, Microarray
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