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KMID : 1034820090050020120
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Molecular & Cellular Toxicology
2009 Volume.5 No. 2 p.120 ~ p.125
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Alaria esculenta Extract Protects against Oxidative Damage by Inducing Heme Oxygenase-1 Expression via Akt and Nrf2
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Choi Chun-Yeon
Jo Guk-Heui
Lee Jung-Im
Seo Young-Wan
Han Tae-Jun
Choi Il-Whan
Liu Kwang-Hyeon
Oh Sang-Taek
Kim Dong-Eun
Jang Won-Hee
Seog Dae-Hyun
Park Yeong-Hong
Yea Sung-Su
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Abstract
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Alaria esculenta is a brown seaweed found in the Arctic. This study investigated the protective effect of A. esculenta extract (AEE) against oxidant-mediated injury and its mode of action in RAW264.7 macrophages. The methyl thiazolyl tetrazolium (MTT) assay showed that treatment reduced cell viability, whereas AEE protected cells from -mediated cytotoxicity in a dose-dependent manner. Because heme oxygenase-1 (HO-1) is known to protect cells against oxidative damage, we investigated the effect of AEE on HO-1 gene expression and HO enzyme activity. The protective effect of AEE against -induced injury was correlated with increased HO enzyme activity. AEE also induced HO-1 mRNA and protein expression, as determined RT-PCR and Western blotting, respectively. To characterize the mechanisms by which AEE induces HO-1 gene expression, we examined the effect of AEE on the nuclear translocation of NF-E2-related factor-2 (Nrf2) and Akt phosphorylation. AEE treatment activated upstream signaling for HO-1 gene expression, including the nuclear translocation of Nrf2 and Akt phosphorylation. Collectively, these results suggest that AEE has anti-oxidant activity that is mediated, at least in part, via the activation of Nrf2 and Akt and the subsequent induction of HO-1 gene expression.
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KEYWORD
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Alaria esculenta, Heme Oxygenase-1, Nrf2, Akt
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