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KMID : 1034820090050020126
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Molecular & Cellular Toxicology
2009 Volume.5 No. 2 p.126 ~ p.132
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Antimutagenic Effects of Ginsenoside Rb, Rg in the CHO-K1 Cells by Benzo[a]pyrene with Chromosomal Aberration Test and Comet Assay
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Kim Jong-Kyu
Kim Soo-Jin
Rim Kyung-Taek
Cho Hae-Won
Kim Hyeon-Yeong
Yang Jeong-Sun
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Abstract
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The usage and types of chemicals are advancing, specializing, large-scaled increasing, and new chemical exposed workers are concerning to occupational disease. The generation of reactive oxygen in the body from carcinogen, mutation and DNA damage in cancer is protected by natural antioxidants (phytochemicals) with antimutagenic effect. There were many reports of ginsenoside Rb, Rg grievances of the genetic mutation to suppress the effect confirm the genetic toxicity test with chromosomal aberration test and the Comet (SCGE) assay confirmed the suppression effect occurring chromosomal DNA damage. We had wanted to evaluate the compatibility and sensitivity between the chromosomal aberration (CA) test and the Comet assay. We used the CA test and Comet assay to evaluate the anti-genotoxicity of ginsenoside Rb and Rg, in CHO-K1 (Chinese hamster ovary fibroblast) cell in vitro, composed negative control (solvent), positive control (benzo[a]pyrene), test group (carcinogen+variety concentration of ginsenoside) group. The positive control was benzo[a]pyrene (50 M), well-known carcinogen, and the negative control was the 1 % DMSO solvent. The test group was a variety concentration of ginsenoside Rb, Rg with 10%, 10%, 10%, 10%, 1%, 10%. In chromo-somal aberration test, we measured the number of cells with abnormally structured chromosome. In Comet assay, the Olive tail moment (OTM) and Tail length (TL) values were measured. The ratio of cell proliferation was increased 8.3% in 10%, 10%, 10%, 10%, 1%, 10% Rb treated groups, and increased 10.4% in 10%, 10%, 10%, 10%, 10%, 1% Rg treated groups. In the CA test, the number of chromosomal aberration was decreased all the Rb and Rg treated groups. In the Comet assay, the OTM values were decreased in all the Rb and Rg treated groups. To evaluate the compatibility between CA and Comet assay, we compared the reducing ratio of chromosomal abnormalities with its OTM values, it was identified the antimutagenicity of ginsenoside, but it was more sensitive the CA test than the Comet assay. Ginsenoside Rb and Rg significantly decrease the number of cells with chromosomal aberration, and decrease the extent of DNA migration. Therefore, ginsenoside Rb, Rg are thought as an antioxidant phytochemicals to protect mutagenicity. The in vitro Comet assay seems to be less sensitive than the in vitro chromosomal aberration test.
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KEYWORD
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Mutagenicity, Genotoxicity, Phytochemicals, Ginsenoside Rb, Rg, Benzo[a]pyrene, Chromosomal aberration (CA), Comet assay, Olive tail moment (OTM), Tail length (TL)
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