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KMID : 1034820090050040283
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Molecular & Cellular Toxicology
2009 Volume.5 No. 4 p.283 ~ p.290
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Heterologous Microarray Hybridization Used for Differential Gene Expression Profiling in Benzo[a]pyrene-exposed Marine Medaka
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Woo Seon-Ock
Won Hyo-Kyoung
Jeon Hye-Young
Kim Bo-Ra
Lee Taek-Kyun
Park Hong-Seog
Yum Seung-Shic
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Abstract
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Differential gene expression profiling was performed in the hepatic tissue of marine medaka fish (Oryzias javanicus) after exposure to benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), by heterologous hybridization using a medaka cDNA microarray. Thirty-eight differentially expressed candidate genes, of which 23 were induced and 15 repressed (P<0.01), were identified and found to be associated with cell cycle, development, endocrine/reproduction, immune, metabolism, nucleic acid/protein binding, signal transduction, or non-categorized. The presumptive physiological changes induced by BaP exposure were identified after considering the biological function of each gene candidate. The results obtained in this study will allow future studies to assess the molecular mechanisms of BaP toxicity and the development of a systems biology approach to the stress biology of organic chemicals.
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KEYWORD
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Marine medaka, Oryzias javanicus, Heterologous hybridization, Microarray benzo[a]pyrene (BaP), Differential gene expression profile
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