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KMID : 1034820100060010033
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Molecular & Cellular Toxicology
2010 Volume.6 No. 1 p.33 ~ p.40
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Uncaria rhynchophylla induces heme oxygenase-1 as a cytoprotective effect in RAW 264.7 macrophages
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Lee Seung-Eun
Ahn Hyun-Jong
Park Cheung-Seog
Lee Sun-Hee
Park Yong-Seek
Lee Nam-Ju
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Abstract
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Uncaria rhynchophylla (UR) is an herbal plant that has been used widely to treat stroke, hypertension and atherosclerosis. Upregulated heme oxygenase-1 (HO-1) plays a crucial role in cytoprotection and anti-inflammatory responses in a variety of pathological models. In this study, we investigated upregulation of HO-1 by UR, which is inducible and cytoprotective enzyme in RAW 264.7 macrophages. Upregulation of HO-1 and signal pathways were examined by western blots, RT-PCR and immunofluorescence staining. Treatment of UR induced HO-1 protein expression and Nrf2 nuclear translocation in RAW 264.7 macrophages. HO-1 induction was strongly inhibited by the suppression of p38 MAPK and ERK activity. Additionally, rottlerin, an inhibitor of protein kinase Cδ(PKC δ), abolished the upregulation of HO-1 protein levels. From the above results, it is suggested that UR may exert a cytoprotective effect via the upregulation of HO-1 in RAW 264.7 macrophages.
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KEYWORD
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Uncaria rhynchophylla, Heme oxygenase 1, Macrophage, Nrf2
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