KMID : 1034820100060020209
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Molecular & Cellular Toxicology 2010 Volume.6 No. 2 p.209 ~ p.215
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Induction of heme oxygenase-1 by acrolein mediates a cytoprotective effect in HepG2 cells
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Song Ju-Young
Lee Seung-Eun Lee Nam-Ju Park Yong-Seek Park Cheung-Seog Ahn Hyun-Jong
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Abstract
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Acrolein is an alpha, beta-unsaturated aldehyde present in cigarette smoke, and is also a product of lipid peroxidation. Heme oxygenase-1 (HO-1) plays critical roles in preventing oxidative stress and other cellular functions, and induces viability and proliferation of tumor cells. Acrolein is well-known to induce HO-1, although the signal pathway has not been fully elucidated. This study elucidated the involved signaling and acrolein¡¯s cytoprotective effects in human hepatocellular carcinoma (HepG2) cells. Cells were treated with acrolein to induce HO-1, whose expression was measured by Western blot, RT-PCR and immunofluorescence staining analyses. Low concentrations of acrolein remarkably increased HO-1 mRNA and protein levels. Acrolein-mediated HO-1 induction was notably decreased by rottlerin and SB203580, selective inhibitors of PKC-¥ä and p38 MAPK, respectively. Furthermore, the cells displayed an increased arrest at the G0/G1 phase of the cell-cycle. The data indicates that acrolein enhances HO-1 expression via PKC-¥ä and p38 MAPK signaling. Acrolein may provide a cytoprotective effect via the expression of HO-1 in HepG2 cells.
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KEYWORD
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Acrolein, Heme oxygenase-1, Human hepatocellular carcinoma, Cytoprotective
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