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KMID : 1034820100060030219
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Molecular & Cellular Toxicology
2010 Volume.6 No. 3 p.219 ~ p.227
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Characterization of gene expression regulated by Panax ginseng in SH-SY5Y cells
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Kim Hak-Jae
Kang Sung-Wook
Kim Mi-Ja
Kim Young-Ock
Kim Geum-Soog
Park Yoon-Hyung
Chung Joo-Ho
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Abstract
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To investigate the effects of Panax ginseng (PG) on SH-SY5Y human neuroblastoma cells, we profiled gene expressions of both normal and PG-treated groups using cDNA microarray. We performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays to determine the effect on PG-induced cytotoxicity. The gene expression profiles in normal group were compared to PG-treated group using 24K human cDNA microarray. MTT assay showed that PG was not cytotoxic at the concentrations of 1?100 μg/mL in SH-SY5Y cells. Consequently, 210 genes exhibited differential expression levels between normal and PG-treated groups through cDNA microarray analysis, and it was found that 52 of the 210 genes were up-regulated while 146 of them were down-regulated by two-fold. PG may induce changes in the expression of genes in SH-SY5Y cells possibly relevant to signal transduction and cellular development. These results help to further understand the molecular framework of PG as a signal transduction inducer of neuronal cells.
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KEYWORD
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Panax ginseng, SH-SY5Y, cDNA microarray
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