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KMID : 1034820100060040359
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Molecular & Cellular Toxicology
2010 Volume.6 No. 4 p.359 ~ p.369
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Identification of atrophy-related proteins produced in response to cast immobilization in rat gastrocnemius muscle
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Kim Jung-Hwan
Kim Bo-Kyung
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Abstract
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Prolonged disuse of skeletal muscle causes atrophy, which is a universal phenomenon induced by various factors such as cast immobilization and space flight. However, the identity of proteins produced in atrophying skeletal muscle cells is poorly understood, especially during a period of cast immobilization. In the present study, we used 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry to investigate protein expression in rat gastrocnemius subjected to cast immobilization for 7 and 21 days. Gastrocnemius muscle mass was lost continuously throughout the 21 days of cast immobilization. Proteomic analysis of silver-stained gels of whole-protein extracts from rat gastrocnemius muscle strips detected 48 proteins. Of these proteins, the expression of 6 proteins changed during cast immobilization; these proteins are involved in metabolic, contraction, and chaperone activities. These results suggest that cast immobilization-induced skeletal muscle atrophy is related to changes in the defense and contractile apparatus proteins in rat gastrocnemius muscle.
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KEYWORD
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Muscle atrophy, Cast immobilization, Gastrocnemius muscle, Proteomics
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