KMID : 1034820110070040339
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Molecular & Cellular Toxicology 2011 Volume.7 No. 4 p.339 ~ p.346
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Gene expression profiling of hair-dying agent, para-phenylenediamine, in human keratinocytes (HaCaT) cells
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So Young-Jin
Shin Chan-Young Song Mee Rha Young-Ah Ryu Jae-Chun
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Abstract
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Para-phenylenediamine (PPD) is widely used in a variety of products, including hair dyes. Sensitization to PPD is a well-known cause of allergic contact dermatitis. However, the molecular mechanism of PPD-induced cellular toxicity remains unclear. We performed a genome-wide analysis of transcriptional responses of human HaCaT keratinocytes to an IC20 dose of PPD (60 ¥ìM). PPD downregulated the expression of 650 genes and upregulated the expression of 854 genes in the keratinocytes. PPD-responsive genes were involved in inflammatory responses, responses to oxidative stress, Toll-like receptor signaling, and metabolism of xenobiotics by cytochrome P450. Our results indicate that changes in gene expression are associated with PPD-induced skin toxicity, and the genes identified may be promising biomarkers of PPDinduced skin toxicity. Possible roles of the responsive genes and related pathways in PPD-induced cellular responses are discussed. This study helps to elucidate cellular changes in transcriptional regulation in response to PPD exposure.
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KEYWORD
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Para-phenylenediamine (PPD 1,4-diaminobenzene CAS 106-50-3), Keratinocytes, Microarray, Gene ontology (GO)
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