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KMID : 1034820120080020163
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Molecular & Cellular Toxicology
2012 Volume.8 No. 2 p.163 ~ p.169
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Effect of freezing stress on the proteome expression of Antarctic green microalga
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Choi Kwang-Man
Lee Mi-Young
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Abstract
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The effects of freezing stress on the protein expression profile and enzyme activity change in Antarctic green microalga were examined in this investigation. Antarctic green microalga was cultured at 4°C for optimal growth, and the temperature was reduced near to 0°C in order to apply freezing stress. The resultant differentially expressed proteins induced by freezing stress were analyzed by mass spectrometry following two-dimensional gel electrophoresis (2-DE). In total, about 320 protein spots were detected on the 2-DE gel, and 17 proteins showed a greater than two-fold change in abundance; of these, 5 proteins were up-regulated, and 12 proteins were down-regulated. Moreover, total enzyme activities and isozyme patterns of several antioxidant enzymes, named catalase, superoxide dismutase and aldehyde dehydrogenase were changed upon applying freezing stress.
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KEYWORD
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Freezing stress, Proteomics, Orthologues, Antioxidant enzyme, Antarctic green microalga
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