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KMID : 1034820120080040335
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Molecular & Cellular Toxicology
2012 Volume.8 No. 4 p.335 ~ p.342
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Effects of recombinant hIL-2-Luffin P1 immunotoxin combining with arotinoid ethylester on the proliferation and apoptosis of Hut-78 cells
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Liu Shu-Lei
He Wei
Wang Ru-Peng
Li Zhi
Wu Ju
Zhao Yun
Hu Xiao-Hong
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Abstract
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Prokaryotic expression vectors pET32a (+)-hIL-2-Luffin P1 and pET32a(+)-Luffin P1 were constructed by gene engineering, followed by induced expression, purification and identification of hIL-2-Luffin P1 and Luffin P1. Hut-78 cells were treated with hIL-2-Luffin P1 and arotinoid ethylester at the different concentrations for the different time periods. The proliferation inhibition rate of Hut-78 cells was determined by MTT assay, and early apoptosis of Hut-78 cells was detected. The fusion protein hIL-2-Luffin P1 with high biological activity was obtained. Treatment of Hut-78 cells for 48 h with hIL-2-Luffin P1, and with hIL-2-Luffin P1 and arotinoid ethylester at different concentrations suggested that the cell proliferation inhibition and apoptosis rates were time- and concentration-dependent. The recombinant immunotoxin hIL-2-Luffin P1 was successfully constructed, which provides an experimental basis for further study of the effect of this immunotoxin on cutaneous T cell lymphomas.
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KEYWORD
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Immunotoxin, HIL-2-Luffin P1, T cell lymphoma, Hut-78, Apoptosis
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