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KMID : 1034820120080040367
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Molecular & Cellular Toxicology
2012 Volume.8 No. 4 p.367 ~ p.374
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Acute effects of H2O2 on the Na+-Ca2+ exchanger of rat hepatocytes
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Shin Sun-Young
Choi Sun-Keun
Jang Jun-Hyeog
Suh Chang-Kook
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Abstract
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Na+-Ca2+ exchanger (NCX) plays an important role in regulating intracellular Ca2+ concentration ([Ca2+]i) in various excitable cells as well as nonexcitable cells. In hepatocytes, however, the role of NCX under oxidative stress is not well understood. The expression and physiological activity of NCX in isolated hepatocytes were examined by molecular and electrophysiological methods. Pretreatments with antisense oligodeoxynucleotides (AS-oligos) depressed the expression of NCX in hepatocytes as well as physiological activities of NCX, measured by membrane current and increase of intracellular Ca2+ concentration ([Ca2+]i) due to the reverse mode of NCX in the hepatocytes. In hepatocytes exposed to 0.1 mM hydrogen peroxide (H2O2), which did not yield an increase in expression of NCX protein, membrane current via NCX and influx of Ca2+ through the reverse mode of NCX were significantly increased. These results suggest that the reverse mode of NCX may participate in Ca2+ overload in hepatocytes under acute oxidative stress.
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KEYWORD
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Na+-Ca2+ exchanger (NCX), Rat hepatocytes, Ca2+, Hydrogen peroxide (H2O2)
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