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KMID : 1034820120080040401
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Molecular & Cellular Toxicology
2012 Volume.8 No. 4 p.401 ~ p.406
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Alteration of discoidin domain receptor-2 expression: possible role in peroxynitrite-induced apoptosis in human cerebral vascular smooth muscle cells
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Li Lin
Yue Zhiyong
Wan Xiaojuan
Zhang Guodong
Song Shasha
Bai Xiaohui
Jiao Yulian
Ju Yuanrong
Li Jianfeng
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Abstract
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Peroxynitrite(ONOO?), a product of nitric oxide and superoxide anion interaction, is a powerful and versatile oxidant. In this work, the human cerebral vascular smooth muscle cells (HCVSMCs) were cultured in vitro and subjected to different concentrations of ONOO?. Cell viability and morphological changes were measured by methylthiazoletetrazolium (MTT) assay and Ho33342/PI double staining respectively. The apoptotic rate was assessed by flow cytometry. The alterations in the expression of DDR2 at both mRNA and protein levels were examined by real-time PCR and Western-blot simultaneously. Direct exposure of HCVSMCs to ONOO? was able to inhibit the cell proliferation, which was further revealed via the apoptotic pathway. ONOO? exerted a dual-effect on DDR2 expression at both mRNA and protein levels that depended on the concentrations of ONOO?. Our results demonstrate for the first time that DDR2 gene may play an important role in ONOO?-induced apoptosis in HCVSMCs.
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KEYWORD
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Peroxynitrite, Discoidin domain receptor 2, Human cerebral vessel smooth muscle cell, Apoptosis
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