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KMID : 1034820130090010029
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Molecular & Cellular Toxicology
2013 Volume.9 No. 1 p.29 ~ p.36
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Cytotoxicity of recombinant immunotoxin containing lectin A chain from Korean mistletoe
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Cho Joon-Seok
Kim In-Bo
Jeong Ju-Seong
Jung Seung-Pil
Kang Tae-Bong
Kim Jong-Bae
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Abstract
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Toxin moiety in immunotoxin (IT) plays an important role in cytotoxicity of target cells. We constructed a recombinant immunotoxin (RIT), which is composed of a recombinant lectin A chain from Korean mistletoe (AKM) and a single-chain Fv (scFv) of an anti-carcinoembryonic antigen (CEA) monoclonal antibody. This single-chain IT was expressed in E. coli, purified, and refolded as a soluble protein. The resulting recombinant protein displayed strong cytotoxicity against CEA-expressing cancer cells but only minimal cytotoxicity against non CEA-expressing cells. The present study, we suggest that AKM may be a potential toxin candidate for development of an IT.
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KEYWORD
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Carcinoembryonic antigen, Korean mistletoe, Lectin, A chain, Cytotoxicity, Immunotoxin
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