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KMID : 1034820130090020195
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Molecular & Cellular Toxicology
2013 Volume.9 No. 2 p.195 ~ p.202
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Effects of PDGF-BB and b-FGF on the production of cytokines, hyaluronic acid and the proliferation of orbital fibroblasts in thyroid ophthalmopathy
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Kang Sung-Mo
Lee So-Yeon
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Abstract
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The effects of Platelet-derived growth factor-BB(PDGF-BB) and Basic-fibroblast growth factor (b-FGF) on the proliferation and production of hyaluronic acid and, cytokine productions in cultured human orbital fibroblasts were evaluated. Orbital fibroblasts were cultured (5 from eyes with thyroid-associated ophthalmopathy and, 5 from normal eyes) and classified into 3 groups-: (1) the Unstimulated group, (2) the Platelet-derived growth factor-BB stimulation group and, (3) the b-Fibroblast growth factor stimulation group. The hyaluronic acid concentration of each group was measured by ELISA. The cytokine production (IL-1β, IL-2, IL-6, IL-8 and RANTES) of each group was measured in the cultured medium. The proliferation of orbital fibroblasts was measured in each group using a thymidine analog EdU, which can be incorporated into DNA during active DNA synthesis. In conclusion, PDGF-BB and b-FGF induced orbital fibroblasts increased production of hyaluronic acid in thyroid-associated ophthalmopathy. In addition to the induction of orbital fibroblast proliferation, PDGF-BB increased the production of IL-8. Further studies on the inhibitory factors of orbital fibroblasts with a larger sample size are warranted to aid in the development of fundamental treatments for thyroid-associated ophthalmopathy.
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KEYWORD
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Thyroid-associated ophthalmopathy, Orbital fibroblast, Platelet-derived growth factor-BB, Basic fibroblast growth factor
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