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KMID : 1034820130090040335
Molecular & Cellular Toxicology
2013 Volume.9 No. 4 p.335 ~ p.340
Chloroform induces HIF-1¥á-dependent VEGF expression in human keratinocytes
Nam Jae-Jun

Lee Ha-Na
Bae Hyun-Cheol
Kim Jin-Hee
Jeong Sang-Hoon
Ryu Woo-In
Son Sang-Wook
Abstract
Vascular endothelial growth factor (VEGF), a primary angiogenic factor, can be expressed under various pathological conditions. We showed that VEGF was upregulated when human keratinocytes were treated with the volatile organic compound chloroform. We investigated the regulation of VEGF expression by the transcription factor hypoxia-inducible factor-1¥á (HIF-1¥á). HIF-1¥á, a protein that normally mediates the expression of proteins in hypoxia, controlled VEGF expression in human keratinocytes treated with chloroform. No significant change was seen in HIF-1¥á mRNA level, consistent with previous reports that HIF-1¥á protein is constitutively expressed and degraded. Furthermore, transfection of cells with plasmids that overexpressed HIF-1¥á upregulated VEGF expression. Small interfering RNA against HIF-1¥á suppressed chloroform-induced VEGF expression in keratinocytes, indicating that HIF-1¥á was involved in VEGF expression. Thus, the results of this study indicated that HIF-1¥á mediated the expression of VEGF in human keratinocytes treated with chloroform.
KEYWORD
Vascular endothelial growth factor, Hypoxia-inducible factor-1¥á, Chloroform, Human keratinocytes
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