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KMID : 1034820140100020149
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Molecular & Cellular Toxicology
2014 Volume.10 No. 2 p.149 ~ p.156
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Gene expression analysis reveals a functional role for the Ag-NPs-induced Egr-1 transcriptional factor in human keratinocytes
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Yoo Gyeong-Min
Jeong Sang-Hoon
Ryu Woo-In
Lee Ha-Na
Kim Jin-Hee
Bae Hyun-Cheol
Son Sang-Wook
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Abstract
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Silver nanoparticles (Ag-NPs) have wellknown antimicrobial properties and have been widely applied as nano-strategies in myriad medical fields. Indeed, early growth response (Egr-1) is an important transcription factor that has been implicated in numerous inflammatory diseases. The administration of Ag-NPs transiently increased the levels of Egr-1 mRNA in human keratinocytes with maximal induction at the 30 minute time point. In an effort to identify molecular signatures associated with cellular responses to Ag-NPs, we adopted an approach involving gene expression profiling of human keratinocytes treated with or without Egr-1 siRNA transfection using a cDNA microarray. The microarray analysis demonstrated that: the expression profile of the genes involved in inflammation and immune response were either stimulated or repressed. Overall, this study was sufficient to reliably recognize the engagement of Egr-1-driven molecular signaling pathways in Ag-NPs-treated keratinocytes.
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KEYWORD
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Silver nanoparticles, Early growth response-1, Microarray, Keratinocytes
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