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KMID : 1034820140100020215
Molecular & Cellular Toxicology
2014 Volume.10 No. 2 p.215 ~ p.221
Immobilization of para-nitrobenzyl esterase-CLEA on electrospun polymer nanofibers for potential use in the synthesis of cephalosporin-derived antibiotics
Sekhon Simranjeet Singh

Park Jae-Min
Ahn Ji-Young
Park Tae-Sun
Kwon Soon-Dong
Kim Yoon-Chul
Min Ji-Ho
Kim Yang-Hoon
Abstract
The para-nitrobenzyl esterase (PNBE) was successfully immobilized onto electrospun polystyrene/poly(styrene-co-maleic anhydride) (PS/PSMA) nanofibers as cross-linked enzyme aggregates (CLEA). The maximum amount of PNBE immobilized on the PSPSMA nanofibers was determined to be 56.4 mg/g of nanofibers. The variation of pH and temperature has been observed to affect the enzymatic activity of the free and immobilized PNBE-CLEA. The immobilized PNBE-CLEA has been observed to show improved stability as compared to the native one. PNBE-CLEA has been found to retain more than 63% activity after 60 consecutive reuses. Finally, the immobilized PNBECLEA presented a high decomposition efficiency of cephalexin tosylate PNB ester. The durability determination of PNBE-CLEA immobilized nanofibers showed 70% of de-esterification after 10 cycles. These results demonstrate that PNBE-CLEA immobilized nanofiber could be used as an economical and environment friendly system for the catalytic process of paranitrobenzyl esters in pharmaceutical synthesis.
KEYWORD
Para-nitrobenzyl esterase, Cross-linked enzyme aggregates, Enzyme immobilization, Electrospun PS-PSMA nanofiber, Cephalosporin-derived antibiotics
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