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KMID : 1034820140100040387
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Molecular & Cellular Toxicology
2014 Volume.10 No. 4 p.387 ~ p.391
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ZnO nanoparticle induces apoptosis by ROS triggered mitochondrial pathway in human keratinocytes
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Ryu Woo-In
Park Yoon-Hee
Bae Hyun-Cheol
Kim Jin-Hee
Jeong Sang-Hoon
Lee Ha-Na
Son Sang-Wook
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Abstract
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Zinc Oxide nanoparticles (ZnO NPs) in its small size with large reactive surfaces can lead to toxicological injury by generating reactive oxygen species (ROS) and oxidative stress. Recently, ZnO NPs were shown to play a role in acute or chronic toxicities with mammalian cells, where its mechanism of toxicity is not fully characterized yet. In this study, the potential mechanisms of ZnO NPs in inducing and increasing oxidative stress for causing cellular apoptosis were investigated with human keratinocytes. Indeed, ZnO NPs induced significant intracellular ROS and mitochondrial ROS productions. And it seemed that cellular ROS levels induced by ZnO NPs led to the dissipation of the mitochondrial membrane potentials and elicited the cellular apoptosis. The induced ROS production by ZnO NPs was blocked with chelator treatment, which inhibited the ZnO ion. The present study demonstrates that increased levels of ROS by ZnO NPs cause mitochondrial dysfunction and cellular apoptosis.
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KEYWORD
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Zinc Oxide, Cytotoxicity, Reactive oxygen species, Keratinocyte, Nanoparticle
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