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KMID : 1034820150110010011
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Molecular & Cellular Toxicology
2015 Volume.11 No. 1 p.11 ~ p.17
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Acteoside reduces testosterone by inhibiting cAMP, p450scc, and StAR in rat Leydig cells
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Liu Shuqiang
Zhang Junwen
Li Weixuan
Zhang Tianxiang
Hu Defu
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Abstract
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The present study investigated the in vivo and in vitro effects of acteoside on testosterone production in Sprague-Dawley (SD) rats. The in vivo experiment revealed that acteoside reduced the testosterone level in serum significantly (P<0.05). The in vitro experiment also illustrated that acteoside significantly reduced testosterone production in SD rat Leydig cells in primary culture (P<0.05). Enzyme-linked immunosorbent assay results demonstrated that acteoside significantly reduced the cyclic adenosine 3', 5'-monophosphate (cAMP) level (P<0.05), and Western blot analysis showed that acteoside significantly reduced cholesterol side-chain cleavage enzyme (p450scc) and steroidogenic acute regulatory (StAR) expression (P< 0.05). Hoechst 33342 staining and Western blotting showed that acteoside did not induce apoptosis in Leydig cells. Together, these results suggest that the acteoside-induced reduction in testosterone production in rat may be at least partially due to down-regulation of cAMP, p450scc, and StAR, but not apoptosis.
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KEYWORD
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Acteoside, cAMP, p450scc, StAR, Testosterone, Leydig cells
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