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KMID : 1034820150110030307
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Molecular & Cellular Toxicology
2015 Volume.11 No. 3 p.307 ~ p.314
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Comparison of gene expression patterns from zebrafish embryos between pure silver nanomaterial and mixed silver nanomaterial containing cells of Hydra magnipapillata
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Park Hyung-Geun
Yeo Min-Kyeong
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Abstract
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Silver nanomaterials have potentially toxic effects in aquatic organisms. However, lower toxicity was demonstrated in hydra exposed to high concentrations of silver nanomaterials. Moreover, aggregated nanomaterials were shown to be excreted from hydra. These excreted nanomaterials had an increased particle size and changed particle shapes compared to the native particles. In addition, the changed nanomaterials were shown to convey reduced toxicity. To investigate the effect of hydra extract on changes in nanotoxicity, we formed a mixed silver nanomaterial that included hydra cells. We investigated the nanotoxicity of this mixture on zebrafish embryogenesis, because zebrafish have very high sensitivity to environmental conditions. The survival rate of embryos in the mixed nano groups was higher than that in the pure nano groups. We also compared the gene expression patterns between pure silver nanomaterials and mixed silver nanomaterials in zebrafish embryos using microarray analysis. We identified the metabolic and cellular processes that had altered gene expression. Genes related to apoptosis were overexpressed in the pure silver nano groups, and genes involved in the immune system had lower expression compared to the mixed silver nano groups.
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KEYWORD
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Silver nano tubes, Silver nano colloidals, Gene ontology, Zebrafish embryos, Hydra magnipapillata
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