KMID : 1034820150110040449
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Molecular & Cellular Toxicology 2015 Volume.11 No. 4 p.449 ~ p.455
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Radix ranunculus temate saponins induces apoptosis via the death receptor and mitochondrial pathways in SGC-7901 cells
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Li Mao-Lin
Gu Han-Min Hang Hong-Ya Jiang Yao-Li Jiang Jiao Gu Qian-Na Wu Wen-Yan
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Abstract
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adix ranunculus temate saponins (RRTS), one of the main constituents extracted from the popular traditional Chinese medicine Radix Ranunculi ternati, has been reported to have various biological activities including anti-cancer effect. The aim of this study is to investigate the effect of RRTS on the cell proliferation and apoptosis in human gastric adenocarcinoma SGC-7901 cells. The data showed that exposure to RRTS for 24 h produced cytotoxic effects on SGC- 7901 cells in a dose-dependent manner (with an IC50 value of 21.22¡¾2.76 ¥ìg/mL), which was accompanied by apoptosis induction (from 2.18¡¾0.89% (control) to 63.72¡¾13.16% (100 ¥ìg/mL)). Both the extrinsic or death receptor pathway and the intrinsic or mitochondrial pathway were involved in RRTS-induced apoptosis in SGC-7901 cells. Furthermore, apoptotic signaling induced by RRTS was amplified by cross-link between the two pathways via the signal-integrating protein Bid. In conclusion, our findings contribute to better understanding the molecular mechanism of RRTS¡¯ effect on gastric cancer cells and form the basis of the therapeutic development of RRTS in treating gastric cancer in the future.
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KEYWORD
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Radix ranunculus temate saponins, SGC-7901, Cell apoptosis, Fas/FasL pathway, Mitochondrial pathway
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