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KMID : 1034820160120010007
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Molecular & Cellular Toxicology
2016 Volume.12 No. 1 p.7 ~ p.14
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Identification of a N 7-guanine adduct of 1-bromopropane in calf thymus DNA by mass spectrometry
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Thapa Pritam
Kim Eun-Kyung
Nepal Mahesh Raj
Jeong Ki-Sun
Kang Mi-Jeong
Noh Keum-Han
Lee Sang-Kyu
Jeong Hye-Gwang
Lee Jun-Ho
Jeong Tae-Cheon
Lee Eung-Seok
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Abstract
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As a replacement for chlorofluorocarbons that cause ozone depletion, 1-bromopropane has been widely used in work place. In the present study, the formation of N 7-guanine adduct in DNA by 1-bromopropane was evaluated in vitro to elucidate the possible mechanism of its toxic action. N 7-Propyl guanine was chemically synthesized and structurally characterized by NMR, UV, HPLC, and liquid chromatographyelectrospray ionization mass spectrometry (LC- ESI MS) for using as a reference standard. An incubation of 2?-deoxyguanosine with 1-bromopropane produced N 7-propyl adduct, which was identified by UV, HPLC and ESI-MS. In addition, N7-guanine adduct was also identified from the incorporation of calf thymus DNA with 1-bromopropane at the physiological condition by LC-ESI MS. Furthermore, the production of adduct was proportional to the amounts of 1-bromopropane used. These results indicated that the molecular mechanism underlying toxic effects of 1-bromopropane would be associated with the adduct formation on DNA at least in part.
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KEYWORD
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1-Bromopropane, N7-Propyl guanine, In vitro, Calf thymus DNA, Adduct formation
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