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KMID : 1034820160120010037
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Molecular & Cellular Toxicology
2016 Volume.12 No. 1 p.37 ~ p.44
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Effects of compounds isolated from a Litsea japonica fruit extract on the TNF-α signaling pathway and cell viability
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Won Ji-Yeon
Kim Jung-Eun
Choi Dong-Hwa
Han Min-Woo
Lee Doo-Hyung
Kang Se-Chan
Song Yoon-Jae
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Abstract
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Tumor necrosis factor-α (TNF-α) plays an important role in inflammatory responses. Deregulated activation of TNF-α signaling is associated with various inflammatory diseases. In view of the finding that the seventy percent ethanol extract of Litsea japonica (LJE) interferes with TNF-α-induced NF-κB activation, activity- guided fractionation was performed to identify the active components of the extract. The EtOAc, CH2Cl2 and n-Hexane fractions of LJE induced suppression of inhibitor of κB (IκB) kinase (IKK), p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK), and significant reduction of TNF-α- induced NF-κB activation. From the CH2Cl2 fraction, hamabiwalactone A, hamabiwalactone B, litsenolide B2 and litsenolide C2 were identified to inhibit TNF- α-induced NF-κB activation. The cytotoxic activities of these lactone compounds were additionally evaluated.
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KEYWORD
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Pro-inflammatory cytokine, NF-κB, Litsea japonica
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