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KMID : 1034820160120020133
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Molecular & Cellular Toxicology
2016 Volume.12 No. 2 p.133 ~ p.138
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Lysosome based toxic detection in Saccharomyces cerevisiae using novel portable fluorometer
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Nguyen Ngoc-Tu
Ahn Ji-Young
Bang Seung-Hyuck
Kim Dong-Kwan
Kim Duk-Min
Kim Yang-Hoon
Min Ji-Ho
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Abstract
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Lysosome is an organelle in the cell, commonly used as biomonitoring tool in environmental pollution. In previous studies, the lysosomal proteome profiling in yeast was analyzed in response to different toxic chemicals, such as sodium meta-arsenite and tetracycline. A recombinant yeast contained the specific biomarker found in the previous studies was constructed for toxic detection. We evaluated green fluorescent intensity of the recombinant yeast exposed to toxic chemicals with dose dependent using by a portable kit designed by our laboratory. The results confirmed the potential of both the yeast and the portable kit to detect each of toxic chemical such as heavy metals, pesticides, or pharmaceuticals at an optimal dose that the intensity of fluorescent protein reached peak at. Therefore, the recombinant yeast and portable kit play an important role to detect various toxic chemicals.
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KEYWORD
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Lysosome, Toxic detection, Fluorescent protein, Portable fluorometer, Spectrofluorometer
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