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KMID : 1034820160120040399
Molecular & Cellular Toxicology
2016 Volume.12 No. 4 p.399 ~ p.407
Extract of Rhus verniciflua Stokes enhances Th1 response and NK cell activity
Lee Eui-Joon

Lee Gi-Hyun
Sohn Sung-Hwa
Bae Hyun-Su
Abstract
Rhus verniciflua Stokes (RVS) is a common poison ivy that causes allergic dermatitis. However, it has been used in Korean Medicine as a therapeutic herb, traditionally for the treatment of abdominal masses. Recent studies have demonstrated evidence of anti-tumoral effects by RVS. However, the effect of RVS on tumor-targeting immune cells such as NK cells and helper T cells remains to be verified. The purposes of this study are as follows: (1) determine whether RVS increases NK cell activity and (2) determine whether RVS increases the expression of cytokines, such as IFN-¥ã, TNF-¥á, IL-4, IL-5, and IL-10, from Th1 and Th2 cells. NK cells and CD4 T-cells from the spleens of BALB/c mice were highly purified using magnetic beads. CD4 T-cells were polarized to Th1-cells by rIL-12 and anti-IL-4 antibody or to Th2-cells by rIL-4 and anti-IL-12 antibody. RVS directly increased NK cell activity in a calcein-AM release assay and augmented IFN-¥ã and TNF-¥á secretion from Th1-cells in an ELISA assay. Thus, these results suggest that RVS may stimulate therapeutic immune responses against cancer via NK cells and Th1 cells.
KEYWORD
Rhus verniciflua Stokes, NK cell, Th1-cells
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