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KMID : 1034820170130010059
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Molecular & Cellular Toxicology
2017 Volume.13 No. 1 p.59 ~ p.64
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The effects of methionine on TCE-induced DNA methylation and mRNA expression changes in mouse liver
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Chen Jiahong
Zhang Hang
Aniagu Stanley
Jiang Yan
Chen Tao
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Abstract
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Trichloroethylene (TCE) had been shown to induce aberrant DNA methylation changes in mouse liver, which could be critical in the development of hepatocellular carcinoma. To understand the effect of methionine, a methyl donor, on TCE-induced mouse liver cancer, we exposed B6C3F1 mice to TCE in the presence or absence of methionine. We found that supplementation with methionine attenuated the TCE-induced hypo- and hyper-methylation status of Cdkn1a and Ihh in mouse liver, respectively. The TCE-induced mRNA expression changes of Cdkn1a, Ihh and Jun were also alleviated by methionine. However, methionine supplementation accelerated the TCE-induced mRNA over-expression of Mki67, and enhanced the TCE-induced mRNA down-regulation of Dnmt3a and Tet2. In summary, our results showed that methionine could attenuate at least part of the TCE-induced DNA methylation changes, but the additional gene expression changes induced by supplementary of methionine does not support the assumption that methionine could prevent TCE-induced liver carcinogenesis.
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KEYWORD
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Trichloroethylene, Methionine, DNA Methylation, mRNA, Liver, Cancer
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