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KMID : 1034820170130040405
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Molecular & Cellular Toxicology
2017 Volume.13 No. 4 p.405 ~ p.417
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Fucoidan inhibits lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and zebrafish larvae
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Jeong Jin-Woo
Hwang Su-Jung
Han Min-Ho
Lee Dae-Sung
Yoo Jong-Su
Choi Il-Whan
Cha Hee-Jae
Kim Suhk-Mann
Kim Heui-Soo
Kim Gi-Young
Jeon You-Jin
Lee Hyo-Jong
Park Hwan-Tae
Yoo Young-Hyun
Choi Yung-Hyun
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Abstract
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Fucoidan, a sulfated polysaccharide, is an active component found in various species of seaweed. Although this compound has a strong anti-inflammatory activity, the underlying mechanisms exerted by fucoidan have not been fully elucidated. In the present study, the anti-inflammatory effects of fucoidan on lipopolysaccharide (LPS)-stimulated macrophages and zebrafish larvae were examined. The present data indicated that fucoidan significantly suppressed the secretion of pro-inflammatory mediators including nitric oxide (NO ) and prostaglandin E2 (PGE2), and cytokines, such as tumor necrosis factor-α and interleukin-1β in RAW 264.7 macrophages without any significant cytotoxicity, the protective effects of which were accompanied by a marked reduction in their regulatory gene expression at the transcription levels. Fucoidan also inhibited translocation of the nuclear factor-kappa B from the cytoplasm to the nucleus and attenuated LPS-induced production of intracellular reactive oxygen species (ROS) in RAW 264.7 macrophages. Moreover, fucoidan reduced NO and PGE2 production and ROS accumulation in LPS-stimulated zebrafish larvae, which was associated with a diminished recruitment of neutrophils and macrophages. Based on the results of this study, we suggest that fucoidan has excellent potential as a therapeutic agent for inflammatory disorders.
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KEYWORD
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Fucoidan, Anti-inflammation, ROS, Macrophages, Zebrafish
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