KMID : 1034820180140020233
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Molecular & Cellular Toxicology 2018 Volume.14 No. 2 p.233 ~ p.239
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Proteins isolated of Pueraria Radix possible to cause allergenic react with immunoglobulin E in human sera
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Kim Yu-Mi
Chung Won-Seok Jang Hyeung-Jin
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Abstract
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Backgrounds: Allergies occur when immunoglobulin E (IgE), which is part of the body¡¯s immune system, binds to molecules. A protein in the foods, plants or pollen is usually the problem. This causes the release of inflammatory chemicals such as histamine. There are a few cases where allergies are cause by proteins derived from herbal medicine, but we do not know yet what protein causes the immune react to IgE.
Methods: Here, we demonstrate to confirm if the Pueraria Radix at the proteomic level triggers IgE immunoreactivity. Finally, we present the molecules of the Pueraria Radix-derived protein that trigger the IgE immune response. To prove our hypothesis, we used proteomic tools and SDS-PAGE stained with Coomassie blue and identified proteins using LC-MS/ MS. Proteins were separated from Pueraria Radix and allergic reaction sera were selected.
Results: We found three positive sera that showed an immune response to Pueraria Radix protein. Positive sera demonstrated a similar pattern of IgE reactivity and mostly restricted to a 50-70 kDa band. Therefore, we have identified number of different IgE binding proteins. Therefore, IgE binding proteins were identified such as enolase, hypothetical protein, DING protein and glycosyl transferase.
Conclusion: The results imply that Pueraria Radix can act as an allergen given the identification of IgE binding proteins among the isolated proteins of Pueraria Radix.
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KEYWORD
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Pueraria Radix, Immunoglobulin E, Herbal medicine, Allergy
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