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KMID : 1034820180140030273
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Molecular & Cellular Toxicology
2018 Volume.14 No. 3 p.273 ~ p.282
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Intrinsic toxicity of stable nanosized titanium dioxide using polyacrylate in human keratinocytes
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Koedrith Preeyaporn
Kim Yeo-Jin
Kim Young-Hun
Kang Joo-Hyon
Seo Young-Rok
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Abstract
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Backgrounds: Trends in the use of an anticoagulant as a dispersing stabilizer are addressed. An effective approach to preparing stable nanosized titanium dioxide (nTiO2) for accurate and systematic assessment of nano- toxicity has not been established.
Methods: Among the dispersants tested here, it was found that sodium polyacrylate (PAA) was the most effective dispersant for nTiO2 in culture media. Our study was the first to demonstrate that a stable PAA-dispersed nTiO2 (nTiO2/PAA) suspension showed more toxic than nTiO2 without PAA in human HaCaT keratinocytes.
Results: Initially, MTT results showed that the stable nTiO2/PAA dispersion exhibited significantly greater cytotoxicity than nTiO2 without PAA. In addition, the stable nTiO2/PAA dispersion induced markedly more oxidative stress than nTiO2 without PAA. Importantly, the stable nTiO2/PAA dispersion caused DNA breakage to a greater extent than nTiO2 without PAA.
Conclusion: Our findings indicated that the anti-coagulant PAA is suitable for preparing homologous dispersed nTiO2 under realistic physiological culture test conditions.
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KEYWORD
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Dispersing stabilizer, Cytotoxicity, Genotoxicity, Human keratinocytes, Titanium dioxide nanoparticles
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