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KMID : 1034820190150040443
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Molecular & Cellular Toxicology
2019 Volume.15 No. 4 p.443 ~ p.452
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In vitro estimation of metal-induced disturbance in chicken gut-oviduct chemokine circuit
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Kim Ki-Hyung
Kim Ju-Il
Han Jae-Yong
Moon Yu-Seok
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Abstract
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Backgrounds: Heavy metals affect various processes in the embryonic development. Embryonic fibroblasts (EFs) play key roles in the innate recognition and wound healing in reproductive tissues.
Methods: Based on the relative toxicities of different inorganic metals and inorganic nonmetallic compounds against murine and chicken EF cells, mechanistic estimations were performed based on transcriptomic analyses.
Results: Lead (II) acetate induced preferential injuries in the chicken EF and mechanistic analyses using transcriptome revealed that chemokine receptor-associated events are potently involved in metal-induced adverse actions. As an early sentinel of metal exposure, the precision-cut intestine slices (PCIS) induced the expression of chemokines including CXCLi1 or CXCLi2, which were potent gut-derived factors that activate chemokine receptors in reproductive organs after circulation.
Conclusion: EF-selective metals can be estimated to trigger the chemokine circuit in the gut-reproductive axis of chickens. This in vitro methodology using PCIS-EF culture could be used as a promising alternate platform for the reproductive immunotoxicological assessment.
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KEYWORD
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Heavy metals, Embryonic fibroblasts, PCIS, Chemokine receptor
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