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KMID : 1034820190150040453
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Molecular & Cellular Toxicology
2019 Volume.15 No. 4 p.453 ~ p.458
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Serum metabolomics in mice after paraquat posioning
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Gao Lina
Wang Guang
Yuan Huiya
Xu Enyu
Liu Guojie
Liu Junting
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Abstract
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Backgrounds: Paraquat is toxic to humans and there is no antidote for paraquat poisoning. Moreover, the mechanisms of paraquat toxicity have not been clearly defined.
Methods: In this study, we developed a serum metabolomic method using gas chromatography- mass spectrometry (GC-MS) to evaluate the effects of paraquat poisoning on mice. Pattern recognition analysis, including both principal component analysis and cross orthogonal partial least squares-discriminate analysis revealed that paraquat poisoning induced energy/redox cycle and metabolic perturbations.
Results: Compared with the control group, the levels of creatinine, citric acid, succinic acid, fumaric acid and glycine in the sub acute paraquat poisoning group increased (P < 0.05), while the levels of alpha-Tocopherol, 6-phosphogluconic acid and palmitoleic acid decreased (P < 0.05).
Conclusion: These findings provide an overview of systematic responses to paraquat exposure and metabolomic insight into the toxicological mechanism of paraquat. Our results demonstrate that the metabolomic method based on GC-MS can be a useful tool to elucidate the mechanism of PQ toxicity, and provide a new direction for its clinical diagnosis and treatment.
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KEYWORD
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Paraquat, Toxicology, Metabolomics, Gas chromatography-mass spectrometry
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