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KMID : 1034820200160010073
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Molecular & Cellular Toxicology
2020 Volume.16 No. 1 p.73 ~ p.81
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Hydra protein reduces the toxicity of Ag?PVP nanoparticles in a 3D A549 cell line
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Lee Jin-Ah
Yeo Min-Kyeong
Kim Soon-Seok
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Abstract
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Backgrounds: Our previous work has confirmed that Hydra magnipapillata extract reduces the toxicity of nanomaterials. We investigated the effects of the Hydra protein (HP) and its role in reducing the toxicity of PVP-coated silver nanoparticles (Ag?PVP NPs) using the A549 lung epithelial cell line.
Results: The viability of cells exposed to Ag?PVP NPs or Ag?PVP NPs?+?HP was 77.93?±?6.96% or 95.04?±?9.20%, respectively, indicating increased viability when HP was added. Although Ag?PVP NPs may have caused apoptosis and necrosis in exposed cells, limited effects were observed in the Ag?PVP NPs?+?HP exposure group. There were no effects on gene expression between the control and all experimental groups. A549 cell in 3D cultures was affected less by the toxicity of Ag?PVP NPs than those in 2D cultures.
Conclusion: In conclusion, HP appears to be a non-cytotoxic substance and can reduce the cytotoxicity of Ag nanomaterials.
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KEYWORD
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Hydra proteins, Nano-toxicity reduction, Ag nano-particle, Ag?PVP, A 549 lung epithelial cell
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