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KMID : 1034820200160020103
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Molecular & Cellular Toxicology
2020 Volume.16 No. 2 p.103 ~ p.118
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Molecular hydrogen protects against oxidative stress-induced RAW 264.7 macrophage cells through the activation of Nrf2 and inhibition of MAPK signaling pathway
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Begum Rahima
Kim Cheol-Su
Fadriquela Ailyn
Bajgai Johny
Jing Xingyu
Kim Dong-Heui
Kim Soo-Ki
Lee Kyu-Jae
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Abstract
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Background: Oxidative stress is involved in the development of many inflammatory, metabolic and aging diseases.
Objective: In this study we investigated, the protective effects of H2 on RAW 264.7 macrophage cell against LPS-and H2O2-induced oxidative stress by the inhibition of MAPK pathway and also activate the Nrf2 pathway.
Results: Our results showed H2 increased the macrophage cell proliferation and generated ROS and NO against LPS stimulation to exert an active immune response. Similarly, H2 protected the macrophage cell from H2O2-induced oxidative stress. H2 reduced the LPS-and H2O2-induced inflammatory cytokine production and intracellular calcium influxes. H2 inhibited the LPS-and H2O2-induced phosphorylation of MAPK pathway and its downstream signaling molecules. Furthermore, H2 protected the macrophage cell from mitochondrial apoptosis. H2 increased Nrf2 protein expression indicating its strong anti-oxidative effects against oxidative stress.
Conclusion: Collectively, our results indicate the strong antioxidant role of H2 against LPS-and H2O2-induced oxidative stress on macrophage cells by activating the Nrf2 pathway and inhibiting the MAPK-signaling pathway.
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KEYWORD
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Molecular hydrogen, Oxidative stress, Anti-oxidant, Macrophage, MAPK signaling, Nrf2
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