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KMID : 1034820200160020119
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Molecular & Cellular Toxicology
2020 Volume.16 No. 2 p.119 ~ p.127
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Anti-inflammatory effect of Rosa laevigata extract on in vitro and in vivo model of allergic asthma via the suppression of IgE and related cytokines
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Lee Seung-Hyeon
Choi Seung-Han
Lee In-Seung
Kim Yu-Mi
An Eun-Jin
Jang Hyeung-Jin
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Abstract
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Backgrounds: Exposure to a toxic stress environment leads to excessive inflammatory reactions and induces allergic asthma resulting in airway hyper-responsiveness. We investigated whether Rosa laevigata Michx. (RL) exhibits anti-inflammatory effects related allergic asthma in both an in vitro and in vivo studies.
Methods: To investigate the preventive effect of RL, A549 cells were pretreated with non-toxic doses of RL (500, 1000 μg/mL) and induced by epidermal growth factor (EGF) (10 ng/mL). First, we evaluated cytotoxicity via a MTT assay. The inhibitory effects of NF-κB activity and COX-2 expression were confirmed by a western blot assay. In the in vivo study, BALB/c mice were challenged with regard to ovalbumin via an intraperitoneal injection of RL (50, 100 mg/kg) and were killed to collect bronchoalveolar lavage fluid, lung tissues and blood serum. The number of inflammatory cells, the secretion of IgE and related cytokines were monitored by ELISA and multiplex assays.
Results: RL significantly suppressed NF-κB activity and COX-2 expression levels in EGF-induced A549 cells. In a chronic inflammation mice model, pretreatment of RL attenuated allergic airway inflammation by reducing inflammatory cells, the secretion of IgE and related cytokines in a dose-dependent manner.
Conclusions: The results of this study present the possibility of RL as a therapeutic agent for allergic asthma patients via the suppression of IgE and related cytokines.
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KEYWORD
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Rosa laevigata, Nuclear factor kappa B, IgE, Cytokines, Asthma, Inflammation
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