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KMID : 1034820200160020193
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Molecular & Cellular Toxicology
2020 Volume.16 No. 2 p.193 ~ p.201
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Flavonoids in Resina Draconis protect against pulmonary fibrosis via the TGF-β1/NOTCH1 pathway
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Yang Liteng
Liu Xin
Zhang Ning
Wu Gaohui
Chen Lifang
Xu Jingyi
Ren Xi
Jiang Xiaoming
Huang Zhijing
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Abstract
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Background: It is known that flavonoids in Resina Draconis (FRD) have anti-inflammatory and analgesic effects, but the function and mechanisms of FRD against pulmonary fibrosis remain unknown.
Objective: The study aimed to study the effect and mechanism of FRD on pulmonary fibrosis.
Methods: Pulmonary fibroblasts were isolated and identified. After treatment with transforming growth factor (TGF)-β1 and FRD-containing serum, expressions of TGF-β1, Jagged1, Notch1, alpha-smooth muscle actin, and collagen I were examined using real-time quantitative PCR and Western blot. Besides, the related proteins were verified in rats with bleomycin-induced pulmonary fibrosis.
Results: We successfully isolated and identified pulmonary fibroblasts and proved that FRD-containing serum inhibits proliferation and downregulates Notch1 expression in TGF-β1-induced fibroblasts. Moreover, our results indicate that FRD might alleviate pulmonary fibrosis via the Jagged1/Notch1 signaling pathways in vivo.
Conclusion: Flavonoids in Resina Draconis might play a key role in pulmonary fibrosis via critical pathways, especially the TGF-β1 and NOTCH1 signaling pathways.
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KEYWORD
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Flavonoids in Resina Draconis, Pulmonary fibrosis, TGF?β1, NOTCH1
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