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KMID : 1034820200160030321
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Molecular & Cellular Toxicology
2020 Volume.16 No. 3 p.321 ~ p.330
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Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells
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Adriana-Berenice Peralta-Vega
Alberto Parra-Barrera
del Pilar Ramos-Godinez Maria
Rebeca Lopez-Marure
Jose Arellano-Galindo
Gutierrez-Iglesias Gisela
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Abstract
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Background: Titanium dioxide (TiO2) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO2 is toxic at the nanometric scale.
Objective: To analyze the in vitro toxic effect of TiO2 NPs on mesenchymal stem cells (hMSCs).
Methods: The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO2 NPs. Viability and cell proliferation tests were carried out at 2, 5 and 7 days of exposure. Additionally, the osteogenic and adipogenic differentiation capacity was evaluated.
Results: TiO2 NPs had a perinuclear arrangement and internalized in vesicles into cytosol, diminished significantly the viability (40% and 30% and 30%) and the proliferation cellular (35%, 50% and 55%), differentiation to osteoblast (38%) and adipoblasts (20%).
Conclusion: TiO2 NPs affect viability, proliferation, and differentiation of hMSCs. Our result suggests more regulation of the use of TiO2.
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KEYWORD
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Mesenchymal stem cell, Titanium dioxide, Nanoparticle, Toxicity, Viability, Cell differentiation, Adipoblasts, Osteoblasts
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