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KMID : 1034820200160040477
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Molecular & Cellular Toxicology
2020 Volume.16 No. 4 p.477 ~ p.484
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Decreased plasma α-synuclein in idiopathic Parkinson’s disease patients after adjusting hemolysis factor
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Shim Kyu-Hwan
Kim Seung-Chan
Youn Young-Chul
Sung Young-Hee
An Seong-Soo A.
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Abstract
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Background: Lewy bodies are pathological hallmarks for the diagnosis of Parkinson’s disease, where the core components are composed of aggregated forms of α-synuclein (α-syn). Although α-syn has been investigated as a potential biomarker for PD, its usage has been limited and still remains controversial.
Objective: An accurate enzyme-linked immunosorbent assay (ELISA) was developed for the detection of α-syn in plasma. The hemolysis score was calculated to eliminate the additive α-syn levels from RBCs. Human plasma samples were collected in heparinized blood tubes from idiopathic Parkinson disease (IPD) and healthy control (HC).
Result: Hemolysis score had a strong correlation with the level of plasma α-syn. From the limited set of samples in this preliminary study, decreased α-syn concentrations were observed in patients with IPD in comparison to HC after adjusting for hemolysis factor. Similar results with a commercial ELISA kit were found for measuring α-syn from the same set of samples with lower correlation and the reduced accuracy of diagnosis than the current study.
Conclusion: The adjustments for the hemolysis factor would be indispensable, supporting plasma α-syn as a potential surrogate biomarker for distinguishing IPD from HC.
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KEYWORD
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Parkinson’s disease, α-Synuclein, Hemolysis
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