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KMID : 1034820210170010001
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Molecular & Cellular Toxicology
2021 Volume.17 No. 1 p.1 ~ p.13
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The pine wood nematode Bursaphelenchus xylophilus and molecular diagnostic methods
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Lee Jin-Pyo
Sekhon Simranjeet Singh
Kim Ji-Hun
Kim Sun-Chang
Cho Byung-Kwan
Ahn Ji-Young
Kim Yang-Hoon
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Abstract
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Background: Bursaphelenchus xylophilus, commonly known as the pine wood nematode (PWN), is one of the most destructive nematode species. It causes pine wilt, a slow progressive disease that results in economic loss worldwide.
Objective: Here, we review the nematode detection methods, including morphological, molecular, chemical, and protein-based methods, and assess how the nematode’s distribution, virulence, incidence, and severity may be attenuated.
Results: Pathogenicity of the pine wood nematode is determined via its morphological and molecular characteristics. Many studies have used nucleic acid PCR amplification as a molecular-based technique, but there are often problems with DNA isolation. Currently, molecular-, chemical-, and protein-based analysis methods are used to diagnose emerging pine wilt diseases.
Conclusion: It is important to quickly diagnose and treat symptomatic trees, but the asymptomatic trees also require quick diagnosis and removal. For this reason, morphological- and DNA-based methods should be combined with chemical- and protein-based approaches for quick and efficient detection.
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KEYWORD
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Pine wilt disease, Bursaphelenchus xylophilus, Diagnosis
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