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KMID : 1034820210170030305
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Molecular & Cellular Toxicology
2021 Volume.17 No. 3 p.305 ~ p.314
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The role of pulmonary ORCC and CLC-2 channels in the response to oxidative stress
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Canella Rita
Benedusi Mascia
Martini Marta
Guiotto Anna
Cervellati Franco
Valacchi Giuseppe
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Abstract
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Background: Exposure of human lung epithelial cells to the oxidant pollutant ozone (O3) alters cell Cl? currents inducing an outward rectifier effect. Among the various Cl? channels, ClC-2 and ORCC seemed to be involved in this response.
Objectives: To identify the channel related to O3 induced current changes.
Results: Down regulating the expression of ORCC and ClC-2 genes and analyzing the membrane current show that the enhancement of the current disappeared when ORCC was silenced. The contribution of ORCC and ClC-2 channels in control and O3 treated cells was obtained by a mathematical approach.
Conclusion: We suggest that O3 activates ORCC channels and slightly inhibited ClC-2 channels in the negative voltage range. These findings open the possibility of identifying the biomolecular changes induced by O3 allowing a possible pharmacological intervention towards chloride current due to oxidative stress.
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KEYWORD
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Human lung epithelial cells, Chloride channels, Ozone, Oxidative stress, Patch clamp technique
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