|
KMID : 1034820210170030347
|
|
Molecular & Cellular Toxicology
2021 Volume.17 No. 3 p.347 ~ p.356
|
|
|
LncRNA taurine up-regulated gene 1 participates in isoflurane induced neurotoxicity
|
|
Zhang Faqiang
Chen Guoqing
Wang Long
Feng Zeguo
Mi Weidong
|
|
|
Abstract
|
|
|
Background: Isoflurane (ISO), with the characteristics of rapid induction and recovery, has become one of the most commonly used inhalation anesthetics in the world.
Objective: The purpose of this study was to explore the biological mechanism of long non-coding RNA taurine up-regulated gene 1 (TUG1) in isoflurane (ISO) induced neurotoxicity through in vitro cell experiments.
Result: ISO exposure inhibited cell viability while promoted ROS generation, cell apoptosis and inflammatory cytokines release in HT22 cells via upregulating long noncoding RNA (lncRNA) TUG1. MiR-15a-5p was a direct target of TUG1 and was reversed regulated by TUG1. Overexpression of miR-15a-5p alleviated neurotoxicity induced by ISO exposure, while downregulation of TUG1 alleviated the neurotoxicity induced by ISO exposure via upregulation of miR-15a-5p.
Conclusion: Downregulation of TUG1 reduced ISO-induced ROS generation, neuron cell apoptosis and inflammatory response. ISO-induced neurotoxicity in HT22 cells was regulated by TUG1/miR-15a-5p axis.
|
|
|
KEYWORD
|
|
|
Isoflurane, Neurotoxicity, MiR-15a-5p, Reactive oxygen species, Taurine up-regulated gene 1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|