|
KMID : 1034820210170040429
|
|
Molecular & Cellular Toxicology
2021 Volume.17 No. 4 p.429 ~ p.438
|
|
|
Association of DNA damage with vitamin D and hair heavy metals of obese women
|
|
Ng Chiat Yin
Amini Farahnaz
Bustami Normina Ahmad
Tan Eugenie Sin Sing
Tan Pui Yee
Mitra Soma Roy
|
|
|
Abstract
|
|
|
Background: Obesity has been linked to DNA damage. The modifiable risk factors may modulate the impact of obesity on DNA damage.
Objective: This study aimed to assess DNA damage and its association with dietary nutrient, serum 25-hydroxyvitamin D (25(OH)D) and concentration of hair heavy metals of obese and non-obese women.
Method: A case?control study was conducted involving 134 women aged between 20 and 50 years. Serum 25(OH)D, fasting glucose, and lipid profile were assessed. Indicators of DNA damage such as percentage of tail DNA, tail moment, tail olive moment, tail intensity and tail length were measured using an alkaline-comet assay. Concentrations of hair heavy metals were quantified using inductively coupled plasma?mass spectrometry (ICP?MS). Participants' daily energy, macro, and micronutrient intake were collected using the Food Frequency Questionnaire.
Results: Mean values of serum 25(OH)D was 31.8?±?0.9 nmol/L. 96.3% of participants were vitamin D deficiency (?0.05). Obese women with serum 25(OH)D level of?≥?31 nmol/L had a significantly lower tail moment (p?=?0.029) and tail olive moment (p?=?0.031); thus, indicating less DNA damage. Additionally, obese women with hair chromium concentration of?≥?5.88 mg/kg had a significantly higher tail moment (p?=?0.047), indicating more DNA damage.
Conclusion: DNA damage among obese women correlated with serum 25(OH)D and hair chromium.
|
|
|
KEYWORD
|
|
|
DNA damage, Heavy metals, Nutrients, Obese, Vitamin D
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|