|
KMID : 1034820220180030285
|
|
Molecular & Cellular Toxicology
2022 Volume.18 No. 3 p.285 ~ p.297
|
|
|
Extracellular polysaccharides purified (Polycan) from Aureobasidium pullulans SM?2001 improves pathophysiology of dystrophin-deficient mdx mice
|
|
Kim Young-Suk
Lim Jong-Min
Shin Jae-Suk
Kim Hyun-Jun
Park Kwang-Il
Oh Tae-Woo
|
|
|
Abstract
|
|
|
Background: Duchenne muscular dystrophy is a hereditary muscular disease involving degeneration (i.e. atrophy and loss of muscle fibres) of skeletal muscles, including the diaphragm, and progressively severe functional decline. A previous study shows Polycan, a type of β-glucan derived from the black yeast Aureobasidium pullulans (SM-2001), promotes osteogenicity and bone loss, and possesses anti-inflammatory activity to induce inflammatory cytokines in human immune and cancer cells.
Objective: In this study, we evaluated changes in exercise load behaviour measurements and changes in muscle-related physiological indicators following oral administration of Polycan in mdx mice, an experimental animal model of Duchenne muscular dystrophy.
Result: In mdx mice, Polycan prevented weight loss and thickness of skeletal muscle. In addition, by monitoring increases in running time of mice on treadmills and performing a grip strength test, we confirmed reduced muscle function was recovered to some extent after administering Polycan to mdx mice. In addition, we confirmed that Polycan significantly altered mRNA expression in a concentration-dependent manner, whereby myogenic transcription factors (MyoD, Myf5 and Myogenin) increased and FoxO3α, MuRF1 and Atrogin-1 decreased. We aimed to investigate the mechanism of action in Polycan on energy metabolism of p-AMPK, SIRT1 and PGC1α with apoptosis expression levels as factors related to signalling pathways. Expression ratios of cleaved-caspase-3/caspase-3 and Bax/Bcl-2 in the Polycan extract-administered group increased compared with the control group.
Conclusion: These results demonstrate that Polycan can improve and protect muscle atrophy by preventing apoptosis via pathway regulation related to myogenic transcription factors and energy metabolism in mdx mice.
|
|
|
KEYWORD
|
|
|
Duchenne muscular dystrophy (DMD), Aureobasidium pullulans (SM-2001), β-glucan, Mdx mice, Anti-atrophic effect
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|